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Image Search Results
Journal: Journal of vascular research
Article Title: Myeloid lineage of human endothelial outgrowth cells circulating in blood and vasculogenic endothelial-like cells in the diseased vessel wall.
doi: 10.1159/000226226
Figure Lengend Snippet: Fig. 1. Characterisation of EOC. FACS analysis of haematopoietic, angioblastic and myeloid markers. EOC ( a ) expression of monocytic markers CD68, Mac-1 and to a lesser extent CD14, but lack of the pan- leukocyte marker CD45. EOC were posi- tive for Flk1, eNOS, VECAD and Tie-2 ( a ). In contrast, MNC were negative for endo- thelial markers eNOS, VECAD and Tie-2 ( b ). c Analysis of endothelial marker tran- script expression in EOC, SOC and HAEC by RT-PCR.
Article Snippet: The primary antibodies used were mouse monoclonal antibodies against Mac-1 (CD11b, Ancell, 159-020), CD31 (Dako, M0823), CD34-PerCP (for FACS, BD Pharmingen, BDEurope, 345804), CD34 (for immunofluorescence, Santa Cruz, sc-7324), Flk-1 (Santa Cruz, sc-6251), CD45 (Santa Cruz, sc-1178),
Techniques: Expressing, Marker, Reverse Transcription Polymerase Chain Reaction
Journal: Journal of vascular research
Article Title: Myeloid lineage of human endothelial outgrowth cells circulating in blood and vasculogenic endothelial-like cells in the diseased vessel wall.
doi: 10.1159/000226226
Figure Lengend Snippet: Fig. 2. a Primary outgrowth colony forma- tion following single-cell deposition, ex- panding colony and confluent monolayer by phase contrast microscopy. b FACS analysis showed positivity with endothe- lial markers (eNOS, VECAD, Tie-2 and Flk-1) and some myeloid markers (CD68 and Mac-1). G3PDH shows equivalent RNA loading conditions.
Article Snippet: The primary antibodies used were mouse monoclonal antibodies against Mac-1 (CD11b, Ancell, 159-020), CD31 (Dako, M0823), CD34-PerCP (for FACS, BD Pharmingen, BDEurope, 345804), CD34 (for immunofluorescence, Santa Cruz, sc-7324), Flk-1 (Santa Cruz, sc-6251), CD45 (Santa Cruz, sc-1178),
Techniques: Microscopy
Journal: Journal of vascular research
Article Title: Myeloid lineage of human endothelial outgrowth cells circulating in blood and vasculogenic endothelial-like cells in the diseased vessel wall.
doi: 10.1159/000226226
Figure Lengend Snippet: Fig. 4. Immunostaining of CD68 and costaining of CD68 with endothelial-specific antigens in microvessels within the adventi- tia of diseased coronary arteries (please note: colours are shown in the online version only). a Immunostaining of CD68+ cells (ar- row; red) lining adventitial microvessel. Note erythrocytes (white; open arrowhead) within the microvessel lumen (L). b Multiple CD68+ cells (red) in microvessels within the adventitia of a me- dium-sized diseased artery. c Recipient-derived Y-chromosome- positive (green dot in blue nucleus) CD68+ (red) cell in adventitial microvessel (arrow) of a vasculopathic coronary artery from a pa-
Article Snippet: The primary antibodies used were mouse monoclonal antibodies against Mac-1 (CD11b, Ancell, 159-020), CD31 (Dako, M0823), CD34-PerCP (for FACS, BD Pharmingen, BDEurope, 345804), CD34 (for immunofluorescence, Santa Cruz, sc-7324), Flk-1 (Santa Cruz, sc-6251), CD45 (Santa Cruz, sc-1178),
Techniques: Immunostaining, Derivative Assay
Journal: Journal of vascular research
Article Title: Myeloid lineage of human endothelial outgrowth cells circulating in blood and vasculogenic endothelial-like cells in the diseased vessel wall.
doi: 10.1159/000226226
Figure Lengend Snippet: Fig. 5. Double immunostaining of CD68 and Tie-2 in cells lining microvascular channels within the adventitia and the lu- men (L) of medium-sized diseased arteries (please note: colours are shown in the on- line version only). a Longitudinal axis of a microvascular channel (asterisks) in dis- eased vessel adventitia showing costaining of CD68 (green) and Tie-2 (red). The merged image shows combined CD68 and Tie-2 staining (yellow). b Transverse sec- tion through multiple microvascular channels in diseased artery adventitia showing similar CD68/Tie-2 costaining. c Erythrocyte (white; open arrowhead)- filled channel in longitudinal orientation within the adventitia of atherosclerotic vessel showing lining cells co-immunola- belling for CD68 and Tie-2. d , e Mouse (MIgG) and rabbit IgG (RIgG) control an- tibody labelling showing lack of immu- noreactivity. f Double immunolabelling (merged-yellow) of CD68 (green) and Tie- 2 (red) in cells lining the endoluminal sur- face of atherosclerotic vessel. In each pan- el, arrows indicate cells that label positive for CD68 and Tie-2 singly and combined.
Article Snippet: The primary antibodies used were mouse monoclonal antibodies against Mac-1 (CD11b, Ancell, 159-020), CD31 (Dako, M0823), CD34-PerCP (for FACS, BD Pharmingen, BDEurope, 345804), CD34 (for immunofluorescence, Santa Cruz, sc-7324), Flk-1 (Santa Cruz, sc-6251), CD45 (Santa Cruz, sc-1178),
Techniques: Double Immunostaining, Staining, Control
Journal: Journal of vascular research
Article Title: Myeloid lineage of human endothelial outgrowth cells circulating in blood and vasculogenic endothelial-like cells in the diseased vessel wall.
doi: 10.1159/000226226
Figure Lengend Snippet: Fig. 6. FISH for Y chromosome indicated that CD68/Tie-2 double-positive cells were of recipient in origin. [Online version: red = CD68 and X chromosome (arrow- head); green = Tie-2 and Y chromosome (open arrowhead); blue = DAPI-stained nuclei; yellow = merged red (CD68) and green (Tie-2)]. The recipient-derived male cells are diploid as indicated by two sex chromosomes (arrow) and show no evi- dence of cell fusion. L = Lumen.
Article Snippet: The primary antibodies used were mouse monoclonal antibodies against Mac-1 (CD11b, Ancell, 159-020), CD31 (Dako, M0823), CD34-PerCP (for FACS, BD Pharmingen, BDEurope, 345804), CD34 (for immunofluorescence, Santa Cruz, sc-7324), Flk-1 (Santa Cruz, sc-6251), CD45 (Santa Cruz, sc-1178),
Techniques: Staining, Derivative Assay